Expression of T4 Early Genes 62, 44, 45 and 46 in the -T4 Recombinant Phage 806-17
نویسندگان
چکیده
منابع مشابه
In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62.
We have developed an in vitro complementation assay for six T4 bacteriophage gene products believed to be components of the T4 DNA replication apparatus. This assay is based upon the fact that DNA synthesis in an infected cell lysate that lacks a given gene product is specifically stimulated by addition of the missing product. By the use of such an assay, two proteins that appear to be the prod...
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The td, nrdB, and sunY introns of bacteriophage T4 each contain a long open reading frame (ORF). These ORFs are preceded by functional T4 late promoters and, in the case of the nrdB intron ORF, a functional middle promoter. Expression of phage-encoded intron ORF-lacZ fusions indicates that these T4 genes are highly regulated. The lack of translation of these ORFs from early pre-mRNAs can be acc...
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Genes 16 and 17 of phage T4 encode two proteins, gp (gene product) 16 and gpl7, required to translocate phage T4 DNA into the head (1, 2, 3). We have determined the DNA sequence of these genes from random and restriction T4 fragments cloned into M13 or various plasmids. The two genes overlap (out-offrame) as indicated. The molecular weights of the proteins, deduced from the DNA sequence (18,367...
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Self-disruptive Escherichia coli that produces foreign target protein was developed. E. coli was co-transformed with two vector plasmids, a target gene expression vector and a lysis gene expression vector. The lytic protein was produced after the expression of the target gene, resulting in simplification of the cell disruption process. In this study, the expression of cloned T4 phage gene e or ...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1985
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-66-6-1215